header.scroll
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+ tweet.png
+ caption The tweet that kicked off this project.
tweet.png
history.scroll
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- 1/27/2025 - first confocal flurescence
+ 1/27/2025 - first confocal fluorescence
history.scroll
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+ 1/28/2025 - MitoView™ Mitochondrial Dyes - Trial size (50 ug), MitoView™ Green ordered
+ 1/27/2025 - first confocal flurescence
latest.scroll
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- *Latest Update:* janus green experiments. fluorescence microscopy on monday.
+ *Latest Update:* [x] janus green experiments. [x] fluorescence microscopy.
latest.scroll
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+ *Next steps:* mitotracker + fluorescence + confocal. Try with buccal cells and WBC. If success, try with 10 subjects.
+
images.scroll
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+ cheek5.jpg
+ caption Cheek cell. Janus green stain.
cheek5.jpg
images.scroll
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+ cheek3.jpg
+ caption Cheek cell. Janus green stain.
cheek3.jpg
calibration2.jpg
calibration2.png
cal2.png
images.scroll
Changed around line 1
+ calibration2.jpg
+ caption For ballpark scale, overlaid fluorescent light micrograph of cultured human cells (source).
+ https://www.open.edu/openlearn/science-maths-technology/a-tour-the-cell/content-section-4.10 source
calibration2.png
cal2.png
images.scroll
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+ # Images
index.scroll
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- images.scorll
+ images.scroll
index.scroll
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+ images.scorll
images.scroll
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+ calibration.jpg
+ caption Calibrating with a stage micrometer.
calibration.jpg
latest.scroll
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- *Latest Update:* janus green experiments.
+ *Latest Update:* janus green experiments. fluorescence microscopy on monday.
latest.scroll
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- https://www.youtube.com/watch?v=KK0rY4hd3EA
+ https://www.youtube.com/watch?v=KK0rY4hd3EA Added minimap to Mitosite
latest.scroll
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+ *Newest Video:* Added minimap to Mitosite
+ https://www.youtube.com/watch?v=KK0rY4hd3EA
+
history.scroll
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- 1/24/2025 - visit cc micrscopy lab. janus green arrived.
+ 1/24/2025 - visit cc microscopy lab. janus green arrived.
history.scroll
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+ 1/21/2025 - added zoom views to Mitosite
+ https://www.youtube.com/watch?v=KK0rY4hd3EA
latest.scroll
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- *Latest Update:* janus green on the way. still iterating with methlylene blue.
+ *Latest Update:* janus green experiments.
history.scroll
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- 1/23/2025 - visit dlab. first oil immersion photos
- https://www.youtube.com/watch?v=jj2ASXjyaTg
+ 1/23/2025 - visit dlab. first oil immersion session
+ https://www.youtube.com/watch?v=jj2ASXjyaTg first oil immersion session
history.scroll
Changed around line 1
+ 1/24/2025 - visit cc micrscopy lab. janus green arrived.
+ 1/23/2025 - visit dlab. first oil immersion photos
+ https://www.youtube.com/watch?v=jj2ASXjyaTg
origin.scroll
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- link https://warpcast.com/breck/0x323bada5
+ https://warpcast.com/breck/0x323bada5
origin.scroll
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- https://warpcast.com/breck/0x323bada5
+ link https://warpcast.com/breck/0x323bada5
origin.scroll
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+ https://warpcast.com/breck/0x323bada5
index.scroll
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+ history.scroll
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- history.scroll
origin.scroll
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+ # Project origin
origin.scroll
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+ origin.jpg
+ caption The cast that started it.
+ https://warpcast.com/breck/0x323bada5 cast
origin.jpg
origin.scroll
index.scroll
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+ origin.scroll
+ mitocounter.scroll
Changed around line 24: history.scroll
- images.scroll
- mitocounter.scroll
index.scroll
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- mitocounter.scroll
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+ images.scroll
+ mitocounter.scroll
about.scroll
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-
- powerhouse.jpg
- width 600px
latest.scroll
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- * *Latest Update*: now attempting to make as much progress with readily available methlyene blue and live image processing
- images.scroll
+ *Latest Update:* janus green on the way. still iterating with methlylene blue.
+
+ *Newest Image:*
+
+ mb.jpg
+ caption Cheek cells. dye: 1% methylene blue.
todo.scroll
Changed around line 2
+ [x] methlyne blue
+ [x] order janus green
+ [] try janus green
- [] Build ScrollSet of mito populations of cell types
+ [] Build ScrollSet of mito populations of cell types
+ [] motors to automatically move scope x/y/z?
latest.scroll
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- * *Latest Update*: lab space in san carlos approved! doing safety training 1/15/2025. we will master the current best practice methods as we invent an at-home, non-toxic solution.
+ * *Latest Update*: now attempting to make as much progress with readily available methlyene blue and live image processing
history.scroll
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- 1/15/2025 - MBC visit/training
+ 1/20/2025 - methlyene blue 1% stain images
+ 1/20/2025 - mitosite rev 2
+ 1/16/2025 - named finalist at Nvidia hackathon!
+ 1/16/2025 - started Mitosite at Nvidia hackathon.
+ 1/15/2025 - MBC safety training complete
images.scroll
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- mb.jpeg
+ mb.jpg
images.scroll
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+ mb.jpeg
+ caption Cheek cells. dye: 1% methylene blue.
mb.jpg
header.scroll
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- ../hub.powerhouse.wiki/powerhouseButton.parsers
+ powerhouse.parsers
powerhouse.parsers
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-
- powerhouseButton
powerhouse.parsers
header.scroll
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- ../powerhouse.wiki/powerhouseButton.parsers
+ ../hub.powerhouse.wiki/powerhouseButton.parsers
header.scroll
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- powerhouseButton.scroll
+ ../powerhouse.wiki/powerhouseButton.parsers
+ powerhouseButton
header.scroll
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+ powerhouseButton.scroll
powerhouseButton.scroll
Changed around line 9: powerhouseButtonParser
+
+ powerhouseButton
powerhouseButton.scroll
Changed around line 9: powerhouseButtonParser
-
- powerhouseButton foobar
powerhouseButton.scroll
Changed around line 1
+ powerhouseButtonParser
+ popularity 0.006294
+ description Powerhouse button with mitochondria icon.
+ extends abstractIconButtonParser
+ catchAllAtomType urlAtom
+ string style position:relative;
+ string svg
+ javascript
+ get link() {
+ return "https://powerhouse.wiki"
+ }
+
+ powerhouseButton foobar
powerhouseButton.scroll
history.scroll
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- 1/24/2025 -
+ 1/15/2025 - Published https://receipts.powerhouse.wiki
+ 1/14/2025 - Purchased Powerhouse.wiki ($3.16)
- 12/19/2024 - SWIFT Compound Monocular Microscope SW200DL ordered. $89.99.
+ 12/19/2024 - Purchased AmScope 72 Pre-Cleaned Blank Microscope Slides and 100 22x22mm Square Cover Glass. ($11.99)
+ 12/19/2024 - Purchased Swift 1.3 Megapixel Digital Camera for Microscopes, Eyepiece Mount, USB 2.0 Connection, Color Photography and Video, Windows and Mac Compatible ($48.99)
+ 12/19/2024 - Purchased SWIFT Compound Monocular Microscope SW200DL ordered. ($89.99)
history.scroll
Changed around line 1
+ 1/15/2025 - MBC visit/training
+ 1/24/2025 -
+ 1/09/2025 - First videos
+ 1/09/2025 - MBC demo
+ 12/27/2024 - First sp images
+ 12/17/2024 - MBC tour
latest.scroll
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- * *Latest Update*: first images (next step: figure out mito staining)
+ * *Latest Update*: lab space in san carlos approved! doing safety training 1/15/2025. we will master the current best practice methods as we invent an at-home, non-toxic solution.
slides.scroll
Changed around line 6
- #
Hypothesis: if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
Hypothesis: if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
+ #
Hypothesis: mitchondria population levels reveal macro health trends. if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
Hypothesis: mitchondria population levels reveal macro health trends. if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
slides.scroll
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- #
Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
+ #
Hypothesis: if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
Hypothesis: if mitolevels are as common and easy to measure as body temperature, blood glucose, blood pressure, heart rate, sleep, we can cure/prevent many diseases and health conditions...
slides.scroll
Changed around line 6
- # Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
+ #
Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
slides.scroll
Changed around line 6
+ # Goal: make mitolevels as common a health measure as body temperature, blood glucose, blood pressure, heart rate, sleep...
slides.scroll
Changed around line 4
- # How are your mito levels right now?
+ # How are your mitolevels right now?
slides.scroll
Changed around line 60: status.png
- # Goal: build a public dataset of 1 billion human mito images by end of year.
+ # Phase 1: build a public dataset of 1 billion human mito images by end of year.
+ [] 1 trillion by EOY 2026
+ [] Figure out what "healthy" mitolevels are
+
+ # Phase 2: at home device for measuring mitolevels
+
- # 1 trillion by EOY 2026
slides.scroll
Changed around line 30: writing.png
- ***
-
slides.scroll
Changed around line 18: scholar.png
- # Hmmm...another interesting paper about mitochondria
+ #
Hmmm...another interesting paper about mitochondria
Hmmm...another interesting paper about mitochondria
slides.scroll
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+ # Hmmm...another interesting paper about mitochondria
slides.scroll
Changed around line 18: scholar.png
- # These mitochondria things seem extremely important...
+
+ #
These mitochondria things seem extremely important...
These mitochondria things seem extremely important...
slides.scroll
Changed around line 18: scholar.png
- ***
-
slides.scroll
Changed around line 2
+ ***
+
+ # How are your mito levels right now?
+
+
printSlides.scroll
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- css body { width: 1000px;} h1 { font-size: 300%;}
+ css body { width: 1000px;} h1 { font-size: 300%;} .abstractDinkusParser {page-break-before: always;}
printSlides.scroll
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- theme dark
+ theme prestige
printSlides.scroll
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+ theme dark
+
+ css body { width: 1000px;} h1 { font-size: 300%;}
+
slideshow.scroll
Changed around line 4: theme dark
- css body { width: 700px;} h1 { font-size: 300%;}
+ css body { width: 1000px;} h1 { font-size: 300%;}
slideshow.scroll
Changed around line 2: buildHtml
- css body { width: 800px;} h1 { font-size: 300%;}
-
+
+ css body { width: 700px;} h1 { font-size: 300%;}
+
slideshow.scroll
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+ title Project PowerHouse
+ theme dark
+
+ css body { width: 800px;} h1 { font-size: 300%;}
+
slides.scroll
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- title Project PowerHouse
- theme dark
-
- css body { width: 800px;} h1 { font-size: 300%;}
-
slideshow.scroll
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-
slideshow.scroll
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+ buildHtml
+
- buildHtml
slideshow.scroll
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- slides.scroll
+ slides.scroll
printSlides.scroll
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+ slides.scroll
+ buildHtml
printSlides.scroll
slideshow.scroll
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+ buildHtml
slides.scroll
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+ title Project PowerHouse
+ theme dark
+
+ css body { width: 800px;} h1 { font-size: 300%;}
+
+ ***
+
+ # Note: the following is intentionally unpolished, for discussion purposes only
+
+ ***
+
+ team.jpg
+ width 200px
+
+ scholar.png
+
+ ***
+
+ # Why haven't we solved cancer, brain, and energy diseases?
+
+ ***
+
+ # These mitochondria things seem extremely important...
+
+ ***
+
+ writing.png
+
+ ***
+
+ # _Extremely_ understudied.
+
+ ***
+
+ library.png
+
+ ***
+
+ # We need more mito images!
+
+ # 1,000,000x more!
+
+ # More people
+ # More cell types
+
+ # More frequent
+
+
+ ***
+
+ start.png
+
+ ***
+
+ # 2 hours later
+
+ token.png
+
+ ***
+
+ status.png
+
+ ***
+
+ # Goal: build a public dataset of 1 billion human mito images by end of year.
+ [] 1 million subjects
+ [] 100 images per session
+ [] 10 sessions @ 2 week intervals
+
+ # 1 trillion by EOY 2026
+
+ ***
+
+ # Timeline
+ [] v0 by 1/31
+ [] funding/presales for protoype
+ [] team by 2/28
+ [] hardware product by Q4 2025
+
+ # Basic science
+ [] Using cheap microscope, cheap video cam, and AI to detect mitos without staining
+ [] Need to validate against large dataset
+ [] Stain approach
+ [] a simple, cheap, non-toxic stain
+ [] Non-optical approaches
+ [] 1 bench space & core access to build our validation set
+
+ # Databases
+ [] Mito images
+ [] Define what are "normal" levels
+ [] Mito labs
+ [] Dye database
+ [] Mito researchers
+
+ # Team Building
+ [] Hardware lead
+ [] Wet lab lead
+ [] CEO
+
+ ****
slideshow.scroll
Changed around line 1
- title Project PowerHouse
- buildHtml
- theme dark
+ slides.scroll
-
- css body { width: 800px;} h1 { font-size: 300%;}
-
- ***
-
- # Note: the following is intentionally unpolished, for discussion purposes only
-
- ***
-
- team.jpg
- width 200px
-
- scholar.png
-
- ***
-
- # Why haven't we solved cancer, brain, and energy diseases?
-
- ***
-
- # These mitochondria things seem extremely important...
-
- ***
-
- writing.png
-
- ***
-
- # _Extremely_ understudied.
-
- ***
-
- library.png
-
- ***
-
- # We need more mito images!
-
- # 1,000,000x more!
-
- # More people
- # More cell types
-
- # More frequent
-
-
- ***
-
- start.png
-
- ***
-
- # 2 hours later
-
- token.png
-
- ***
-
- status.png
-
- ***
-
- # Goal: build a public dataset of 1 billion human mito images by end of year.
- [] 1 million subjects
- [] 100 images per session
- [] 10 sessions @ 2 week intervals
-
- # 1 trillion by EOY 2026
-
- ***
-
- # Timeline
- [] v0 by 1/31
- [] funding/presales for protoype
- [] team by 2/28
- [] hardware product by Q4 2025
-
- # Basic science
- [] Using cheap microscope, cheap video cam, and AI to detect mitos without staining
- [] Need to validate against large dataset
- [] Stain approach
- [] a simple, cheap, non-toxic stain
- [] Non-optical approaches
- [] 1 bench space & core access to build our validation set
-
- # Databases
- [] Mito images
- [] Define what are "normal" levels
- [] Mito labs
- [] Dye database
- [] Mito researchers
-
- # Team Building
- [] Hardware lead
- [] Wet lab lead
- [] CEO
-
- ****
slides.scroll
slideshow.scroll
Changed around line 44: library.png
+
slideshow.scroll
Changed around line 46: library.png
+
slideshow.scroll
Changed around line 38: library.png
- # We need more mito images! # 1,000,000x more!
+ # We need more mito images!
+
+ # 1,000,000x more!
+
slideshow.scroll
Changed around line 38: library.png
- # We need more mito images!
- # 1,000,000x more!
+ # We need more mito images! # 1,000,000x more!
slideshow.scroll
Changed around line 39: library.png
-
-
slideshow.scroll
Changed around line 44: library.png
- # Longitudinal!
+ # More frequent
slideshow.scroll
Changed around line 44: library.png
- # and Longitudinal!
+ # Longitudinal!
slideshow.scroll
Changed around line 42: library.png
- ## More people
- ## More cell types
- ## and Longitudinal!
+ # More people
+ # More cell types
+ # and Longitudinal!
slideshow.scroll
Changed around line 42: library.png
- - More people
- - More cell types
- - and Longitudinal!
+ ## More people
+ ## More cell types
+ ## and Longitudinal!
slideshow.scroll
Changed around line 42: library.png
- # From more people, more cell types, and Longitudinal!
+ - More people
+ - More cell types
+ - and Longitudinal!
slideshow.scroll
Changed around line 40: library.png
- # More much!
+ # 1,000,000x more!
slideshow.scroll
Changed around line 22: scholar.png
- # These mitochondria things seem incredibly important...
+ # These mitochondria things seem extremely important...
Changed around line 30: writing.png
- # _Incredibly_ understudied.
+ # _Extremely_ understudied.